Aso has officially confirmed the 22 teams that will compete in the next Tour de France Femmes avec Zwift. In addition to the 15 WorldTour teams - Ag Insurance Soudal Team, Canton//SRAM Xondacrypto, Ceratizit Pro Cycling, FDJ Suez, Fenix Deceuninck, Human Powered Health, Lidl Trek, Liv AlUla Jayco, Movistar Team, Roland, Team Picnic PostNL, Team SD Worx Protime, Team Visma | Lease a Bike, UAE Enirates ADQ and Uno-X Mobility - the top teams from the 2024 season are automatically admitted, namely EF Education Oatly and Volkerwessel Cycling Team.

Invitations have been extended to the French teams Arkea B&B Hotels, Cofidis Women Team, St Michel Preference Home Auber 93 and Winspace Orange Seal, and the Spanish team Laboral Kutxa Fundacion Euskadi.