A thrilling duel hovering around fifty kilometers per hour between Dutch riders dominated the opening, with victory and the first pink jersey leader's bib of the 30th Giro di Toscana-Memorial Michela Fanini going to Lonneke Uneken (the last to start), in the individual time trial prologue of a couple of kilometers on the Chiesina Uzzanese circuit. The winner, who traveled at 50 kilometers per hour and wears the jersey of Volkerwessels Cycling Team (the team that won the Giro di Toscana in 2025 with Eline Jansen), prevailed over her two Dutch teammates Souren and Knijnenburg, while another Dutch rider, Kloese, finished fourth. The starting platform was located on Via Veneto (Spanish rider Alonso started first) while the finish line was situated on Via Cavour. The best Italian rider was Lara Crestanello, who finished an excellent seventh.

FINAL STANDINGS: 1) Lonneke Uneken (Volkerwessels NED) 2.030 km, in 2:26.22, average speed 49.980 km/h; 2) Scarlett Souren (id) at 4.36; 3) Anne Knijenburg (id.) at 7.43; 4) Kloese at 7.48; 5) Sharp at 7.68. A total of 157 riders from 27 teams are competing in the race organized by S.C. Michela Fanini. Tomorrow's second stage is suited for sprinters, running from Serravalle Pistoiese to Quarrata, two locations beloved by the Giro. The stage covers 112 kilometers with the gathering on Via Garibaldi and the official start at 1:00 PM from Via Lucchese. The finish is on Via Montalbano in the center of Quarrata between 3:30 PM and 4:00 PM.